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This web application is dedicated to a G protein-coupled receptor (GPCR) for ADP (P2Y12). The database stores three data types, i) mutant evolutionary variations derived from 77 P2Y12 sequences (orthologs), ii) experimental functional characterization of all 19 possible variants at 77 contiguous positions (1463 mutants) and iii) structural information through P2Y12 homology models. The web-application provides several tools for browsing, analysing and comparing the experimental dataset by combining structural information and evolutionary sequence variation. 3D visualization is accomplished by a Jmol applet.

receptor

P2Y12 mutant library...

  • enables the comparison of in vivo (evolutionary conservation of P2Y12 orthologs) and in vitro findings (site-saturation mutagenesis, conservation determined in functional assays)
  • provides analyses of data by: focused extraction, comparison, projection and mapping on three-dimensional receptor structures and models.

Features...

  • Multiple sequence alignment of all species is linked to the functional results
  • The interactive 3D Search allows selecting particular amino acid positions by providing the user with information about available side-chain variations
  • Distribution analysis of natural variants for each amino acid in the human P2Y12 sequence
  • 2D - Snake-Plot - Search representing the requested mutation data in a two-dimensional plot of the P2Y12 sequence and hyperlinking those to the detailed mutation information
  • Combined search at multiple sites for gathering information about multi-site-specific sequence variations

Citation...

If you use and publish results extracted from the P2Y12 receptor Mutagenesis Database, we acknowledge for citing:

Coester M, Wittkopf D, Kreuchwig A, Kleinau G, Thor D, Krause G, Schoeneberg T (2012) Using ortholog sequence data to predict the functional relevance of mutations in G-protein-coupled receptors. FASEB J. 2012 Aug;26(8):3273-81.