About GPCR-SSFE
The GPCR-Sequence-Structure-Feature-Extractor (SSFE) database provides template suggestions and homology models of the helical regions of 5025 family A GPCRs. SSFE is based on our published workflow for identifying key sequence and structural motifs in family A GPCRs which is used to guide template selection and build homology models.
Users can access the models stored in GPCR-SSFE by either browsing the family (subdivided into 19 groups) or by searching for results using a UniProt identifier. Users can also enter a GPCR sequence of their choice for analysis by GPCR-SSFE. Results returned include the suggestion of which templates to use for modelling, sequence alignments and homology models of the predicted helical regions of the query GPCR.
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The overall topology of the templates is remarkably similar, with the transmembrane helices superimposing relatively well in most cases. hAA2AR (PDB entry ) is represented in orange, tB1AR in blue (PDB entry ), hB2AR in green (PDB entry ), sRho in yellow (PDB entry ) and bRho in red (PDB entry ). The image was rendered using Pymol. |
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| Please click here to view the five superimposed GPCR structures in 3D using Jmol. | ||||
References
Worth CL, Kleinau G and Krause G (2009) Comparative sequence and structural analyses of G-protein-coupled receptor crystal structures and implications for molecular models. PLoS One 4(9): e7011.