PostHeaderIcon About GPCR-SSFE

The GPCR-Sequence-Structure-Feature-Extractor (SSFE) database provides template suggestions and homology models of the helical regions of 5025 family A GPCRs. SSFE is based on our published workflow for identifying key sequence and structural motifs in family A GPCRs which is used to guide template selection and build homology models.

Users can access the models stored in GPCR-SSFE by either browsing the family (subdivided into 19 groups) or by searching for results using a UniProt identifier. Users can also enter a GPCR sequence of their choice for analysis by GPCR-SSFE. Results returned include the suggestion of which templates to use for modelling, sequence alignments and homology models of the predicted helical regions of the query GPCR.

Picture of superimposed GPCR structures
Structural superimposition of the five available GPCR structures

The overall topology of the templates is remarkably similar, with the transmembrane helices superimposing relatively well in most cases. hAA2AR (PDB entry 3EML) is represented in orange, tB1AR in blue (PDB entry 2VT4), hB2AR in green (PDB entry 2RH1), sRho in yellow (PDB entry 2Z73) and bRho in red (PDB entry 1U19). The image was rendered using Pymol.

Please click here to view the five superimposed GPCR structures in 3D using Jmol.

PostHeaderIcon References

Worth CL, Kreuchwig A, Kleinau G, Krause G (2011) GPCR-SSFE: a comprehensive database of G-protein-coupled receptor template predictions and homology models. BMC Bioinformatics. 2011 May 23;12:185.

The methodology used for template selection is detailed in the following paper:
Worth CL, Kleinau G and Krause G (2009) Comparative sequence and structural analyses of G-protein-coupled receptor crystal structures and implications for molecular models. PLoS One 4(9): e7011.