A Homology Modeling Resource for G-Protein Coupled Receptors

About GPCR-SSFE 2.0

The GPCR-Sequence-Structure-Feature-Extractor (SSFE) web server and database provides template suggestions and homology models of Class A GPCRs. GPCR-SSFE 2.0 is based on our published workflow for identifying key sequence and structural motifs in Class A GPCRs which is used to guide template selection and build homology models. The methodology has recently been updated to include 27 template structures, uses a new fingerprint correlation scoring strategy and includes loop modelling.

Users can access the models stored in GPCR-SSFE 2.0 by either browsing the superfamily (subdivided into 10 families) or by searching for results using a UniProt identifier. Users can also enter a GPCR sequence of their choice for analysis by GPCR-SSFE 2.0. Results returned include the suggestion of which template(s) to use for modelling, sequence alignments and homology models of the query GPCR.

GPCR-SSFE 2.0 has been updated to include 33 template structures
As of December 2017, five new inactive structures have been added to our pool of templates: CCR2, CCR9, ADORA1, PAR2 and LPAR6. Template selection and modelling has been redone on 1011 GPCR sequences from human, mouse and rat.